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| International Agrophysics |
| publisher: | Institute of Agrophysics
Polish Academy of Sciences
Lublin, Poland |
| ISSN: |
0236-8722 |
vol. 20, nr. 4 (2006)
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Numbers of culturable bacteria in soil under mineral or organic cultivation:
comparison of Hattori's 'FOR' and standard dilution plate methods
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M. Dąbek-Szreniawska
1, M. Hajnos
1, G. Stotzky
2, Y. Collins
2, J. Malicki
3
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1 Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, P.O. Box 201, 20-290 Lublin 27, Poland
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2 New York University, Biology Department, Laboratory of Microbial Ecology, 100 Washington Square East, New York, NY
10003-6688, U.S.A.
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3 Technical University, Nadbystrzycka 40, 20-618 Lublin, Poland
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vol. 20 (2006), nr. 4,
pp. 277-288
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abstract
Two concepts and methods for determining the
number of culturable bacteria were compared in soil under conventional
ie mineral fertilizers, herbicides, fungicides or organic management
ie manure or compost, mechanical or manual weeding.
In the first method, colony-forming units (CFUs) of bacteria were
counted after 14 days of incubation of soil dilutions on two different
media ie diluted nutrient broth agar (DNBA) and soil extract
agar (SEA). In the second method, the First Order Reaction (FOR)
model was used to determine the CFUs of bacteria that were
counted 7 times on successive days after plating the soil dilutions
on the media. The CFUs were also expressed on the basis of 1 g of
fresh soil, 1 g of oven-dry soil, 1 cm3 of soil, 1 cm3 (ml) of soil
dilution, 1 cm3 of soil porosity, and 1 cm2 of pore surface. The
numbers of bacteria were compared with the organic carbon
content of the soils, as soil organic matter was assumed to be the
major substrate for the growth of the bacteria. The content of
carbon was 0.96% (in soil receiving mineral fertilizers) and 1.34%
(in soil receiving organic fertilizers), resulting in a comparative
ratio of 0.96:1.34 = 0.72.
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keywords
soil bacteria, methods of determining bacterial
numbers, first order reaction (FOR) model.
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