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| International Agrophysics |
| publisher: | Institute of Agrophysics
Polish Academy of Sciences
Lublin, Poland |
| ISSN: |
0236-8722 |
vol. 20, nr. 2 (2006)
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Optimization of selenium accumulation in Rhodotorula rubra cells by treatment
of culturing medium with pulse electric field
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| (get PDF )
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U. Pankiewicz, J. Jamroz, A. Schodziński
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Department of Food Quality Evaluation, University of Agriculture, Skromna 8, 20-704 Lublin, Poland |
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vol. 20 (2006), nr. 2,
pp. 147-152
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abstract
Electroporation was used as a method for Rhodotorula rubra biomass enrichment in selenium. The highest selenium accumulation in yeast cells was achieved after 10 min pulse electric field (PEF) exposure of 16 h shaken culture. Multiple PEF exposure of yeast culture did not favour selenium accumulation. Optimization of selenium concentration in a medium resulted in an over two-fold increase of its accumulation in cells. About three-fold increase of dead yeast cells was recorded in analysed range of selenium concentrations.
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keywords
selenium, biomass, Rhodotorula rubra, pulse electric field
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